Achieving Plant CRISPR Targeting that Limits Off‐Target Effects
نویسندگان
چکیده
منابع مشابه
Achieving Plant CRISPR Targeting that Limits Off-Target Effects.
The CRISPR-Cas9 system (clustered regularly interspaced short palindromic repeats with associated Cas9 protein) has been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species; but development of plant genome editing has not yet fully considered potential off-target mismatches that may lead to unintended changes within the genome. ...
متن کاملTracking CRISPR targeting
Over the last few years, CRISPR has become a vital tool for scientists, as the bacterial acquired immunity system has been adapted to function in eukaryotic cells as a means to edit the genome, modulate gene expression, and label chromosomes for live cell imaging. The biochemical activity of CRISPR complexes—consisting of the Cas9 nuclease and an RNA that guides it to fi nd and cleave a specifi...
متن کاملGeographic range limits: achieving synthesis.
Understanding of the determinants of species' geographic range limits remains poorly integrated. In part, this is because of the diversity of perspectives on the issue, and because empirical studies have lagged substantially behind developments in theory. Here, I provide a broad overview, drawing together many of the disparate threads, considering, in turn, how influences on the terms of a simp...
متن کاملCRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA.
Horizontal gene transfer (HGT) in bacteria and archaea occurs through phage transduction, transformation, or conjugation, and the latter is particularly important for the spread of antibiotic resistance. Clustered, regularly interspaced, short palindromic repeat (CRISPR) loci confer sequence-directed immunity against phages. A clinical isolate of Staphylococcus epidermidis harbors a CRISPR spac...
متن کاملCRISPR/Cas-Mediated In Planta Gene Targeting.
The recent emergence of the CRISPR/Cas system has boosted the possibilities for precise genome engineering approaches throughout all kingdoms of life. The most common application for plants is targeted mutagenesis, whereby a Cas9-mediated DNA double-strand break (DSB) is repaired by mutagenic nonhomologous end joining (NHEJ). However, the site-specific alteration of a genomic sequence or integr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Plant Genome
سال: 2016
ISSN: 1940-3372,1940-3372
DOI: 10.3835/plantgenome2016.05.0047